Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-PRKCDBP Polyclonal Antibody, Unconjugated (bs-6085R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat mammary gland); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \\tPRKCDBP Polyclonal Antibody, Unconjugated ( \\tbs-6085R ) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.