Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti ALOX15B/15 Lipoxygenase 2 Polyclonal Antibody, Unconjugated (bs-6335R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 74kD. Observed band size: 74kD.
Paraformaldehyde-fixed, paraffin embedded human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ALOX15B Polyclonal Antibody, Unconjugated (bs-6335R ) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.