Paraformaldehyde-fixed, paraffin embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ZnT-1 Polyclonal Antibody, Unconjugated (bs-6440R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAPI staining
Paraformaldehyde-fixed, paraffin embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ZnT-1 Polyclonal Antibody, Unconjugated (bs-6440R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ZnT-1 Polyclonal Antibody, Unconjugated (bs-6440R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse intestine lysates probed with ZnT-1 Polyclonal Antibody, Unconjugated (bs-6440R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37˚C.