Mouse spleen and kidney lysates probed with IL-4l1 Polyclonal Antibody, unconjugated (bs-6841R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-4I1 Polyclonal Antibody, Unconjugated (bs-6841R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining