Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval with 0.4% pepsin at 37°C for 30min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CLDND2 Polyclonal Antibody, Unconjugated (bs-8014R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining
paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval with 0.4% pepsin at 37°C for 30min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CLDND2 Polyclonal Antibody, Unconjugated (bs-8014R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining
Mouse brain lysates probed with Anti-CLDND2 Polyclonal Antibody, Unconjugated (bs-8014R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.