Mouse liver lysates probed with Rabbit Anti-FAM134C Polyclonal Antibody (bs-8153R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAM134C Polyclonal Antibody, Unconjugated (bs-8153R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAM134C Polyclonal Antibody, Unconjugated (bs-8153R) at 1:400 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) for 90 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAM134C Polyclonal Antibody, Unconjugated (bs-8153R) at 1:400 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) for 90 minutes and DAPI staining of the nuclei.