Antigen: bs-9021P, 0.2ug/100ul \\n\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\n\\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\n\\nTMB staining;\\n\\nRead the data in MicroplateReader by 450
Lane 1: human colon carcinoma lysates Lane 2: mouse intestine lysates probed with Anti IQCJ Polyclonal Antibody, Unconjugated (bs-9021R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 18kD. Observed band size: 18kD.