Western blot analysis of Bcl-2 expression in U-937 (A,D), HL-60 (B,E) and Jurkat (C,F) whole cell lysates. Antibodies tested include Bcl-2 (100): sc-509 (A-C) and Bcl-2 (N-19): sc-492 (D-F).
Bcl-2 (N-19): sc-492. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining (A). Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic localization (B).
Bcl-2 (N-19)-G: sc-492-G. Western blot analysis of Bcl-2 expression in non-transfected: sc-117752 (A) and mouse Bcl-2 transfected: sc-118779 (B) 293T whole cell lysates.
Bcl-2 (N-19)-G: sc-492-G. Western blot analysis of Bcl-2 expression in HL-60 whole cell lysate.
Bcl-2 (N-19): sc-492. Western blot analysis of Bcl-2 expression in non-transfected: sc-117752 (A) and human Bcl-2 transfected: sc-176463 (B) 293T whole cell lysates.