Western blot analysis of Bcl-2 expression in U-937 (A,D), HL-60 (B,E) and Jurkat (C,F) whole cell lysates. Antibodies tested include Bcl-2 (100): sc-509 (A-C) and Bcl-2 (N-19): sc-492 (D-F).
Western blot analysis of Bcl-2 expression in HL-60 (A,D), Jurkat (B,E) and WEHI-231 (C,F) whole cell lysates. Antibodies tested include Bcl-2 (C-2): sc-7382 (A-C) and Bcl-2 (100): sc-509 (D-F).
Bcl-2 (100): sc-509. Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic staining.
Bcl-2 (100): sc-509. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon tissue showing cytoplasmic staining of lymphoid cells.
Bcl-2 (100) PE: sc-509 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-2866.
Bcl-2 (100) Alexa Fluor 488: sc-509 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-3890.
Bcl-2 (100) Alexa Fluor 647: sc-509 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-24636.
Bcl-2 (100) Alexa Fluor 488: sc-509 AF488. Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic localization using indirect Texas Red (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
Bcl-2 (100): sc-509. Western blot analysis of Bcl-2 expression in non-transfected: sc-117752 (A) and human Bcl-2 transfected: sc-176463 (B) 293T whole cell lysates.
Bcl-2 (100) FITC: sc-509 FITC. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, mouse IgG
1: sc-2855.
goat anti-mouse IgG
1-HRP: sc-2969. Western blot analysis of Bcl-2 expression in Jurkat whole cell lysate. Antibody tested: Bcl-2 (100): sc-509.
donkey anti-mouse IgG-HRP: sc-2314. Western blot analysis of Bcl-2 expression in Jurkat whole cell lysate. Antibody tested (IgG
1): Bcl-2 (100): sc-509.
Bcl-2 (100): sc-509. Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp.
goat anti-mouse IgG
1-HRP: sc-2060. Western blot analysis of Bcl-2 expression in HL-60 whole cell lysate. Antibody tested: Bcl-2 (100): sc-509.