Anti-Rab18 (C-terminal) antibody produced in rabbit

Name	Anti-Rab18 (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200174
Prices	$55.60, $322.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human, Mouse, Rat
Antigen	synthetic peptide corresponding to amino acids 177-189 of human Rab18 conjugated to KLH
Description	Rabbit Polyclonal
Gene	RAB18
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>NRK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-Rab18 (C-terminal) (<B>Cat. No. SAB4200174</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (<B>Cat. No. 18772</B>). Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
NRK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-Rab18 (C-terminal) (Cat. No. SAB4200174). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Whole extract of MM3MG cells was separated on SDS-PAGE and probed with Rabbit Anti-RAB18 (C-terminal) (<B>Cat. No. SAB4200174</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2.5 μg/mL antibody<BR/>2. 5 μg/mL antibody<BR/>3. 5 μg/mL antibody + RAB18 (C-terminal) immunizing peptide

Immunoblotting
Whole extract of MM3MG cells was separated on SDS-PAGE and probed with Rabbit Anti-RAB18 (C-terminal) (Cat. No. SAB4200174). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody
3. 5 μg/mL antibody + RAB18 (C-terminal) immunizing peptide

<B>Immunoprecipitation </B><BR/>Rabbit Anti-Rab18 (C-terminal) (<B>Cat. No. SAB4200174</B>) was used to immunoprecipitate Rab18 from PC3 whole cell extract. Detection antibody: Rabbit Anti-Rab18 (<B>Cat. No. SAB4200173</B>).<BR/><B>Lanes</B><BR/>1. 2.5 μg/mL antibody<BR/>2. 5 μg/mL antibody<BR/>3. Negative control: 5 μg/mL non-relevant antibody

Immunoprecipitation
Rabbit Anti-Rab18 (C-terminal) (Cat. No. SAB4200174) was used to immunoprecipitate Rab18 from PC3 whole cell extract. Detection antibody: Rabbit Anti-Rab18 (Cat. No. SAB4200173).
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody
3. Negative control: 5 μg/mL non-relevant antibody