Anti-NCAPH2 (211-225) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: SAB1101634
• Prices: $328.50
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 211-225 of human NCAPH2
• Description: Rabbit Polyclonal
• Gene: NCAPH2
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-NCAPH2 (211-225) (RB): Cat. No. SAB1101634: Immunofluorescence of HUVEC cells using Anti-NCAPH2 (211-225) (RB) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence Graph
Anti-NCAPH2 (211-225) (RB): Cat. No. SAB1101634: Intensity analysis of Anti-NCAPH2 (211-225) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6400) as compared with the negative control.

Immunoblotting
Lysate of HEK-293T cells (lane 1) and HEK-293T cells overexpressing human NCAPH2 (lanes 2-4) were separated on SDS-PAGE, blotted with Anti-NCAPH2 (211-225) (Cat. No. SAB1101634) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500
3. Negative control (only secondary antibody)
4. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μg/mL.