Anti-PTPN3 (477-490) antibody produced in rabbit
| Name | Anti-PTPN3 (477-490) antibody produced in rabbit |
|---|---|
| Supplier | Sigma-Aldrich |
| Catalog | T6453 |
| Prices | $342.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | WB |
| Species Reactivities | Human |
| Antigen | synthetic peptide corresponding to amino acids 477-490 of human PTPN3 |
| Description | Rabbit Polyclonal |
| Gene | PTPN3 |
| Conjugate | unconjugated |
| Supplier Page | Shop |
Product images
ImmunofluorescenceAnti-PTPN3 (477-490) (RB): Cat. No. T6453: Immunofluorescence of HUVEC cells using PTPN3 (477-490) (RB), Cat. No. T6453 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
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ImmunoblottingLysate of HEK-293T cells overexpressing human PTPN3 was separated on SDS-PAGE, blotted with Anti- PTPN3 (477-490) (Cat. No. T6453) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + PTPN3 immunizing peptide (human, 477-490)
3. Antibody dilution 1:1,000
ChartAnti-PTPN3 (477-490) (RB): Cat. No. T6453: Intensity analysis of Anti-PTPN3 (477-490) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.