ImmunofluorescenceMDA-MB-231 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-SSSCA1 Clone: SSSCA1-A206 (
Cat. No. SAB4100182) diluted to 1:100. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingLysate of HEK-293T cells expressing FLAG
®-tagged human SSSCA1 was separated on SDS-PAGE, probed with Mouse Anti-SSSCA1 Clone: SSSCA1-A206 (
Cat. No. SAB4100182). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:2,000
2. Negative control
3. Positive control Anti-FLAG
®
ImmunoblottingMDA-MB-231 cell extract was separated on SDS-PAGE and probed with Mouse Anti-SSSCA1 Clone: SSSCA1-A206 (
Cat. No. SAB4100182). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:1,000
3. Negative control