ImmunoblottingLysate of HEK-293T cells overexpressing human FLAG
®-PPP1R14D fusion protein was separated on SDS-PAGE, blotted with Anti-PPP1R14D (111-124) (
Cat. No. SAB1102537) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + PPP1R14D immunizing peptide (human, 111-124)
3. Negative control (only secondary antibody)
4. Anti-FLAG
® antibody (
Cat. No. F7425), working concentration 2 μg/mL