ImmunofluorescenceCOS7 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton
™ X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-FAM48A/P38IP (
Cat. No. SAB4200382). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (
Cat. No. 18772).
ImmunoblottingWhole extract of HEK-293T cells was separated on SDS-PAGE and probed with Rabbit Anti-FAM48A/P38IP (
Cat. No. SAB4200382). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 1 μg/mL antibody
2. 2 μg/mL antibody
3. 2 μg/mL antibody + FAM48A/P38IP immunizing peptide
ImmunoblottingWhole extract of A20 cells was separated on SDS-PAGE and probed with Rabbit Anti-FAM48A/P38IP (
Cat. No. SAB4200382). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody
ImmunoblottingWhole extract of PC12 cells was separated on SDS-PAGE and probed with Rabbit Anti-FAM48A/P38IP (
Cat. No. SAB4200382). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody