Anti-ZNF746 (C-terminal region) antibody produced in rabbit

Name	Anti-ZNF746 (C-terminal region) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200531
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to a sequence at the C-terminal region of human ZNF746, conjugated to KLH
Description	Rabbit Polyclonal
Gene	ZNF746
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Cell lysates of HEK-293T overexpressing human ZNF746 (lanes 1,3) and HEK293-T non-transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-ZNF746 (C-terminal region) (<B>Cat. No. SAB4200531</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody: 0.1 μg/mL<BR/>2. Antibody: 0.1 μg/mL<BR/>3. Antibody: 0.1 μg/mL + ZNF746 immunizing peptide

Immunoblotting
Cell lysates of HEK-293T overexpressing human ZNF746 (lanes 1,3) and HEK293-T non-transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-ZNF746 (C-terminal region) (Cat. No. SAB4200531) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody: 0.1 μg/mL
2. Antibody: 0.1 μg/mL
3. Antibody: 0.1 μg/mL + ZNF746 immunizing peptide

<B>Immunoprecipitation</B><BR/>Anti-ZNF746 (C-terminal region) (<B>Cat. No. SAB4200531</B>), was used to immunoprecipitate ZNF746 from HEK293-T cells overexpressing human ZNF746. Detection antibody: Anti-ZNF746 (<B>Cat. No. SAB4200530</B>).<BR/><B>Lanes</B><BR/>1. IP antibody: 2 μg<BR/>2. IP antibody: 5 μg<BR/>3. Negative control: without cell lysate<BR/>4. Negative control: without IP antibody

Immunoprecipitation
Anti-ZNF746 (C-terminal region) (Cat. No. SAB4200531), was used to immunoprecipitate ZNF746 from HEK293-T cells overexpressing human ZNF746. Detection antibody: Anti-ZNF746 (Cat. No. SAB4200530).
Lanes
1. IP antibody: 2 μg
2. IP antibody: 5 μg
3. Negative control: without cell lysate
4. Negative control: without IP antibody