Anti-ADD2 (456-470) antibody produced in rabbit

Name	Anti-ADD2 (456-470) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	A7359
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 456-470 of human ADD2
Description	Rabbit Polyclonal
Gene	ADD2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-ADD2 (456-470): <B>Cat. No. A7359</B>: Immunofluorescence of HUVEC cells using ADD2 (456-470), Cat. No. A7359 (red) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-ADD2 (456-470): Cat. No. A7359: Immunofluorescence of HUVEC cells using ADD2 (456-470), Cat. No. A7359 (red) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human ADD2 was separated on SDS-PAGE, blotted with Anti-ADD2 (456-470) (<B>Cat. No. A7359</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + ADD2 immunizing peptide (human, 456-470)

Immunoblotting
Lysate of HEK-293T cells overexpressing human ADD2 was separated on SDS-PAGE, blotted with Anti-ADD2 (456-470) (Cat. No. A7359) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + ADD2 immunizing peptide (human, 456-470)

<B>Chart</B><BR/>Anti-ADD2 (456-470): <B>Cat. No. A7359</B>: Intensity analysis of Anti-ADD2 (456-470) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-ADD2 (456-470): Cat. No. A7359: Intensity analysis of Anti-ADD2 (456-470) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Soluble plasma HLA peptidome as a potential source for cancer biomarkers. - Soluble plasma HLA peptidome as a potential source for cancer biomarkers.

Bassani-Sternberg M, Barnea E, Beer I, Avivi I, Katz T, Admon A. Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18769-76. doi:

Targeted disruption of the beta adducin gene (Add2) causes red blood cell - Targeted disruption of the beta adducin gene (Add2) causes red blood cell

Gilligan DM, Lozovatsky L, Gwynn B, Brugnara C, Mohandas N, Peters LL. Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10717-22.

beta-adducin (Add2) KO mice show synaptic plasticity, motor coordination and - beta-adducin (Add2) KO mice show synaptic plasticity, motor coordination and

Porro F, Rosato-Siri M, Leone E, Costessi L, Iaconcig A, Tongiorgi E, Muro AF. Genes Brain Behav. 2010 Feb;9(1):84-96.