Western blot analysis of unphosphorylated (A,C,E) and human recombinant connexin 43 phosphorylated by rat recombinant ERK 2 (B,D,E). Antibodies tested include connexin 43 (H-150): sc-9059 (A,B), p-connexin 43 (Ser279/282)-R: sc-12900-R (C,D) and p-connexin 43 (Ser 255): sc-12899-R (E,F).
Western blot analysis of connexin 43 phosphorylation in untreated (A, D), PMA treated (B, E) and PMA and lambda protein phosphatase treated (C, F) HEK293 whole cell lysates. Antibodies tested include p-connexin 43 (Ser 279/282)-R: sc-12900-R (A, B, C) and connexin 43 (C-20)-R: sc-6560-R (D, E, F).
Western blot analysis of connexin 43 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) F9 whole cell lysates. Antibodies tested include p-connexin 43 (Ser 279/282)-R: sc-12900-R (A,B) and connexin 43 (C-20)-R: sc-6560-R (C,D).