Monoclonal Anti-DGKB antibody produced in mouse

Name	Monoclonal Anti-DGKB antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4100114
Prices	$358.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	DGKB-A60
Applications	WB
Species Reactivities	Human
Antigen	DGKB purified recombinant protein
Description	Mouse Monoclonal
Gene	DGKB
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-DGKB Clone: DGKB-A60 (<B>Cat. No. SAB4100114</B>) diluted to 1:100. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-DGKB Clone: DGKB-A60 (Cat. No. SAB4100114) diluted to 1:100. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells expressing FLAG<SUP>®</SUP>-tagged human DGKB was separated on SDS-PAGE, probed with Mouse Anti-DGKB Clone: DGKB-A60 (<B>Cat. No. SAB4100114</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250<BR/>2. Negative control<BR/>3. Positive control Anti-FLAG<SUP>®</SUP>

Immunoblotting
Lysate of HEK-293T cells expressing FLAG^®-tagged human DGKB was separated on SDS-PAGE, probed with Mouse Anti-DGKB Clone: DGKB-A60 (Cat. No. SAB4100114). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Negative control
3. Positive control Anti-FLAG^®

<B>Immunoblotting</B><BR/>HeLa cell extract was separated on SDS-PAGE and probed with Mouse Anti-DGKB Clone: DGKB-A60 (<B>Cat. No. SAB4100114</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:2,000<BR/>3. Negative control

Immunoblotting
HeLa cell extract was separated on SDS-PAGE and probed with Mouse Anti-DGKB Clone: DGKB-A60 (Cat. No. SAB4100114). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000
2. Antibody dilution 1:2,000
3. Negative control