Anti-CLGN (46-60) antibody produced in rabbit

Name	Anti-CLGN (46-60) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C7247
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 46-60 of human CLGN
Description	Rabbit Polyclonal
Gene	CLGN
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CLGN was separated on SDS-PAGE, blotted with Anti-CLGN (46-60) (<B>Cat. No. C7247</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human CLGN was separated on SDS-PAGE, blotted with Anti-CLGN (46-60) (Cat. No. C7247) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000

<B>Immunofluorescence</B><BR/>Anti-CLGN (46-60): <B>Cat. No. C7247</B>: Immunofluorescence of HUVEC cells using CLGN (46-60), Cat. No. C7247 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-CLGN (46-60): Cat. No. C7247: Immunofluorescence of HUVEC cells using CLGN (46-60), Cat. No. C7247 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Chart</B><BR/>Anti-CLGN (46-60): <B>Cat. No. C7247</B>: Intensity analysis of Anti-CLGN (46-60) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-CLGN (46-60): Cat. No. C7247: Intensity analysis of Anti-CLGN (46-60) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Cloning and characterization of the human Calmegin gene encoding putative - Cloning and characterization of the human Calmegin gene encoding putative

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Glycan specificity of a testis-specific lectin chaperone calmegin and effects of - Glycan specificity of a testis-specific lectin chaperone calmegin and effects of

Sakono M, Seko A, Takeda Y, Aikawa J, Hachisu M, Koizumi A, Fujikawa K, Ito Y. Biochim Biophys Acta. 2014 Sep;1840(9):2904-13. doi:

Molecular chaperone calmegin localization to the endoplasmic reticulum of meiotic - Molecular chaperone calmegin localization to the endoplasmic reticulum of meiotic

Yoshinaga K, Tanii I, Toshimori K. Arch Histol Cytol. 1999 Aug;62(3):283-93.