Anti-CRISP3 (186-200) antibody produced in rabbit

Name	Anti-CRISP3 (186-200) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C9996
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 186-200 of human CRISP3
Description	Rabbit Polyclonal
Gene	CRISP3
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-CRISP3 (186-200): <B>Cat. No. C9996</B>: Immunofluorescence of HUVEC cells using CRISP3 (186-200) (RB),<B>Cat. No. C9996</B> (red) at a 1:800 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-CRISP3 (186-200): Cat. No. C9996: Immunofluorescence of HUVEC cells using CRISP3 (186-200) (RB),Cat. No. C9996 (red) at a 1:800 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CRISP3 was separated on SDS-PAGE, blotted with Anti-CRISP3 (186-200) (<B>Cat. No. C9996</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human CRISP3 was separated on SDS-PAGE, blotted with Anti-CRISP3 (186-200) (Cat. No. C9996) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-CRISP3 (186-200): <B>Cat. No. C9996</B>: Intensity analysis of Anti-CRISP3 (186-200) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-CRISP3 (186-200): Cat. No. C9996: Intensity analysis of Anti-CRISP3 (186-200) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

The human cysteine-rich secretory protein (CRISP) family. Primary structure and - The human cysteine-rich secretory protein (CRISP) family. Primary structure and

Kratzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD. Eur J Biochem. 1996 Mar 15;236(3):827-36.

Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in human - Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in human

Udby L, Sorensen OE, Pass J, Johnsen AH, Behrendt N, Borregaard N, Kjeldsen L. Biochemistry. 2004 Oct 12;43(40):12877-86.

Cysteine-rich secretory protein 3 overexpression is linked to a subset of - Cysteine-rich secretory protein 3 overexpression is linked to a subset of

Grupp K, Kohl S, Sirma H, Simon R, Steurer S, Becker A, Adam M, Izbicki J, Sauter G, Minner S, Schlomm T, Tsourlakis MC. Mod Pathol. 2013 May;26(5):733-42.

Cysteine-rich secretory protein-3 (CRISP3) is strongly up-regulated in prostate - Cysteine-rich secretory protein-3 (CRISP3) is strongly up-regulated in prostate

Ribeiro FR, Paulo P, Costa VL, Barros-Silva JD, Ramalho-Carvalho J, Jeronimo C, Henrique R, Lind GE, Skotheim RI, Lothe RA, Teixeira MR. PLoS One. 2011;6(7):e22317.

The role of cysteine-rich secretory proteins in male fertility. - The role of cysteine-rich secretory proteins in male fertility.

Koppers AJ, Reddy T, O'Bryan MK. Asian J Androl. 2011 Jan;13(1):111-7.