Anti-α1,2-Mannosidase IA antibody produced in rabbit

Name	Anti-α1,2-Mannosidase IA antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	M3694
Prices	$403.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	ICC/IF WB
Species Reactivities	Human, Mouse, Rat
Antigen	synthetic peptide corresponding to amino acids 138-154 of human α1,2-mannosidase IA with N-terminal added cysteine, conjugated to KLH
Description	Rabbit Polyclonal
Gene	MAN1A1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HepG2 cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 μg/mL Rabbit Anti-α1,2-Mannosidase IA (<B>Cat. No. M3694</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunofluorescence
HepG2 cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 μg/mL Rabbit Anti-α1,2-Mannosidase IA (Cat. No. M3694). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

<B>Immunofluorescence</B><BR/>NRK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 μg/mL Rabbit Anti-α1,2-Mannosidase IA (<B>Cat. No. M3694</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunofluorescence
NRK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 10 μg/mL Rabbit Anti-α1,2-Mannosidase IA (Cat. No. M3694). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

<B>Immunohistochemistry</B><BR/>Anti-α 1,2-Mannosidase IA: <B>Cat. No. M3694</B>: Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded rat pancreas at 0.2 μg/mL.

Immunohistochemistry
Anti-α 1,2-Mannosidase IA: Cat. No. M3694: Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded rat pancreas at 0.2 μg/mL.

Immunohistochemistry
Anti-α 1,2-Mannosidase IA: Cat. No. M3694: Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded rat kidney at 0.2 μg/mL.

Immunohistochemistry
Anti-α 1,2-Mannosidase IA: Cat. No. M3694: Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded mouse testis at 0.4 μg/mL.

Immunohistochemistry
Anti-α 1,2-Mannosidase IA: Cat. No. M3694: Immunoperoxidase staining of Anti-α 1,2-Mannosidase IA in formalin fixed, paraffin-embedded mouse kidney at 0.4 μg/mL.

<B>Immunoblotting</B><BR/>Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-α1,2-Mannosidase IA (<B>Cat. No. M3694</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 1 μg/mL antibody<BR/>2. 1 μg/mL antibody + α1,2-Mannosidase IA immunizing peptide (human, 138-154)

Immunoblotting
Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-α1,2-Mannosidase IA (Cat. No. M3694). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 1 μg/mL antibody
2. 1 μg/mL antibody + α1,2-Mannosidase IA immunizing peptide (human, 138-154)

<B>Immunoblotting</B><BR/>Whole extract of HepG2 cells was separated on SDS-PAGE and probed with 0.5 μg/mL Rabbit Anti-α1,2-Mannosidase IA (<B>Cat. No. M3694</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.

Immunoblotting
Whole extract of HepG2 cells was separated on SDS-PAGE and probed with 0.5 μg/mL Rabbit Anti-α1,2-Mannosidase IA (Cat. No. M3694). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.