ImmunohistochemistryPurkinje cell localization of VILIP-3 in human cerebellum. Formalin-fixed tissue was stained with 6 μg/mL Anti-VILIP-3 (
Cat. No. SAB4200281) followed by Donkey Anti-Rabbit IgG, biotin conjugate and by Streptavidin-HRP conjugate (×40 magnification).
ImmunofluorescenceCytoplasmic localization of VILIP-3 in PC-12 cells. Fixed cells were stained with 5 μg/mL Anti-VILIP-3 (
Cat. No. SAB4200281) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunoblottingCell and tissue extracts were separated on SDS-PAGE, blotted with 1 μg/mL Anti-VILIP-3 (
Cat. No. SAB4200281), and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Rat brain (S1 fraction)
2. Mouse brain (S1 fraction)
3. PC-12
4. HEK293-T overexpressing human VILIP-3
5. HEK293-T non-transfected
6. Mouse brain (S1 fraction), Antibody + immunizing VILIP-3 peptide