Monoclonal Anti-SORBS2 antibody produced in mouse

Name	Monoclonal Anti-SORBS2 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4200183
Prices	$55.60, $322.00
Sizes	25 µl, 200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	S5C
Applications	ICC/IF IP WB
Species Reactivities	Human, Hamster, Dog, Rat, Mouse
Antigen	mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 546-562 of human SORBS2, conjugated to KLH
Description	Mouse Monoclonal
Gene	SORBS2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% v/v Triton<SUP>™</SUP> X-100. Fixed cells were stained with 10 μg/mL Mouse Anti-SORBS2 Clone:S5C (<B>Cat. No. SAB4200183</B>). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cell nuclei were stained with DAPI.

Immunofluorescence
HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% v/v Triton^™ X-100. Fixed cells were stained with 10 μg/mL Mouse Anti-SORBS2 Clone:S5C (Cat. No. SAB4200183). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cell nuclei were stained with DAPI.

<B>Immunoblotting</B><BR/>Lysates were separated on SDS-PAGE and probed with 3 μg/mL Mouse Anti-SORBS2 Clone: S5C (<B>Cat. No. SAB4200183</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. L929; 2. 293T; 3. HeLa; 4. HepG2; 5. MCF7; 6. NRK; 7. 3T3; 8. MDCK; 9. CHO

Immunoblotting
Lysates were separated on SDS-PAGE and probed with 3 μg/mL Mouse Anti-SORBS2 Clone: S5C (Cat. No. SAB4200183). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. L929; 2. 293T; 3. HeLa; 4. HepG2; 5. MCF7; 6. NRK; 7. 3T3; 8. MDCK; 9. CHO

<B>Immunoprecipitation </B><BR/>Mouse Anti-SORBS2 antibodies (<B>Cat. No. SAB4200177</B> and <B>SAB4200183</B>) were used to immunoprecipitate SORBS2 from HeLa whole cell extract. Detection antibody: Rabbit Anti-SORBS2 antibody (<B>Cat. No. 4200037</B>)<BR/><B>Lanes</B><BR/>1. 15 μg antibody Mouse Anti-SORBS2 Clone S13N (<B>Cat. No. SAB4200177</B>)<BR/>2. 15 μg antibody Mouse Anti-SORBS2 Clone S5C (<B>Cat. No. SAB4200183</B>)<BR/>3. Negative control: without antibody<BR/>4. Positive control: HeLa cell extract

Immunoprecipitation
Mouse Anti-SORBS2 antibodies (Cat. No. SAB4200177 and SAB4200183) were used to immunoprecipitate SORBS2 from HeLa whole cell extract. Detection antibody: Rabbit Anti-SORBS2 antibody (Cat. No. 4200037)
Lanes
1. 15 μg antibody Mouse Anti-SORBS2 Clone S13N (Cat. No. SAB4200177)
2. 15 μg antibody Mouse Anti-SORBS2 Clone S5C (Cat. No. SAB4200183)
3. Negative control: without antibody
4. Positive control: HeLa cell extract

Product References

ArgBP2 and the SoHo family of adapter proteins in oncogenic diseases. - ArgBP2 and the SoHo family of adapter proteins in oncogenic diseases.

Roignot J, Soubeyran P. Cell Adh Migr. 2009 Apr-Jun;3(2):167-70. Epub 2009 Apr 5.

Cbl-ArgBP2 complex mediates ubiquitination and degradation of c-Abl. - Cbl-ArgBP2 complex mediates ubiquitination and degradation of c-Abl.

Soubeyran P, Barac A, Szymkiewicz I, Dikic I. Biochem J. 2003 Feb 15;370(Pt 1):29-34.

ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl-interacting protein, - ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl-interacting protein,

Wang B, Golemis EA, Kruh GD. J Biol Chem. 1997 Jul 11;272(28):17542-50.