Anti-PCBP4 (256-270) antibody produced in rabbit

Name	Anti-PCBP4 (256-270) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C1122
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 256-270 of human PCBP4
Description	Rabbit Polyclonal
Gene	PCBP4
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-PCBP4 (256-270): <B>Cat. No. C1122</B>: Immunofluorescence of HUVEC cells using PCBP4 (256-270) (RB),<B>Cat. No. C1122</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-PCBP4 (256-270): Cat. No. C1122: Immunofluorescence of HUVEC cells using PCBP4 (256-270) (RB),Cat. No. C1122 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human PCBP4 was separated on SDS-PAGE, blotted with Anti-PCBP4 (256-270) (<B>Cat. No. C1122</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250

Immunoblotting
Lysate of HEK-293T cells overexpressing human PCBP4 was separated on SDS-PAGE, blotted with Anti-PCBP4 (256-270) (Cat. No. C1122) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250

<B>Chart</B><BR/>Anti-PCBP4 (256-270): <B>Cat. No. C1122</B>: Intensity analysis of Anti-PCBP4 (256-270) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-PCBP4 (256-270): Cat. No. C1122: Intensity analysis of Anti-PCBP4 (256-270) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is - MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is

Zhu J, Chen X. Mol Cell Biol. 2000 Aug;20(15):5602-18.

Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its - Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its

Pio R, Zudaire I, Pino I, Castano Z, Zabalegui N, Vicent S, Garcia-Amigot F, Odero MD, Lozano MD, Garcia-Foncillas J, Calasanz MJ, Montuenga LM. Cancer Res. 2004 Jun 15;64(12):4171-9.

Expression of alpha CP-4 inhibits cell cycle progression and suppresses - Expression of alpha CP-4 inhibits cell cycle progression and suppresses

Castano Z, Vergara-Irigaray N, Pajares MJ, Montuenga LM, Pio R. Int J Cancer. 2008 Apr 1;122(7):1512-20.