Monoclonal Anti-NEZHA antibody produced in mouse

Name	Monoclonal Anti-NEZHA antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4200415
Prices	$335.50
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	NEZHA-1
Applications	IP ICC/IF WB
Species Reactivities	Human, Dog
Antigen	synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH
Description	Mouse Monoclonal
Gene	CAMSAP3
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Whole extract of SW480 was separated on SDS-PAGE and probed with Monoclonal Anti-NEZHA Clone: NEZHA-1 (<B>Cat. No. SAB4200415</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 2.5 μg/mL antibody<BR/>2. 5 μg/mL antibody

Immunoblotting
Whole extract of SW480 was separated on SDS-PAGE and probed with Monoclonal Anti-NEZHA Clone: NEZHA-1 (Cat. No. SAB4200415). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody

<B>Immunofluorescence</B><BR/>MDCK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-NEZHA Clone: NEZHA-1 (<B>Cat. No. SAB4200415</B>). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
MDCK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-NEZHA Clone: NEZHA-1 (Cat. No. SAB4200415). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoprecipitation </B><BR/>Monoclonal Anti-NEZHA Clone: NEZHA-1 (<B>Cat. No. SAB4200415</B>) was used to immunoprecipitate NEZHA from a whole extract of HEK-293T cells overexpressing human NEZHA. Detection antibody: Rabbit Anti-NEZHA (C-terminal) (<B>Cat. No. SAB4200171</B>).<BR/><B>Lanes</B><BR/>1. 5 μg antibody<BR/>2. 10 μg antibody<BR/>3. Negative control: 10 μg non-relevant antibody<BR/>4. Negative control: no cell extract<BR/>5. Whole extract of HEK-293T cells overexpressing human NEZHA

Immunoprecipitation
Monoclonal Anti-NEZHA Clone: NEZHA-1 (Cat. No. SAB4200415) was used to immunoprecipitate NEZHA from a whole extract of HEK-293T cells overexpressing human NEZHA. Detection antibody: Rabbit Anti-NEZHA (C-terminal) (Cat. No. SAB4200171).
Lanes
1. 5 μg antibody
2. 10 μg antibody
3. Negative control: 10 μg non-relevant antibody
4. Negative control: no cell extract
5. Whole extract of HEK-293T cells overexpressing human NEZHA