Anti-PFKFB4 (1-15) antibody produced in rabbit

Name	Anti-PFKFB4 (1-15) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	F2806
Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 1-15 of human PFKFB4
Description	Rabbit Polyclonal
Gene	PFKFB4
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-PFKFB4 (1-15): <B>Cat. No. F2806</B>: Immunofluorescence of HUVEC cells using PFKFB4 (1-15) (RB),<B>Cat. No. F2806</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-PFKFB4 (1-15): Cat. No. F2806: Immunofluorescence of HUVEC cells using PFKFB4 (1-15) (RB),Cat. No. F2806 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human PFKFB4 was separated on SDS-PAGE, blotted with Anti-PFKFB4 (1-15) (<B>Cat. No. F2806</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human PFKFB4 was separated on SDS-PAGE, blotted with Anti-PFKFB4 (1-15) (Cat. No. F2806) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-PFKFB4 (1-15): <B>Cat. No. F2806</B>: Intensity analysis of Anti-PFKFB4 (1-15) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-PFKFB4 (1-15): Cat. No. F2806: Intensity analysis of Anti-PFKFB4 (1-15) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress - PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress

Novellasdemunt L, Bultot L, Manzano A, Ventura F, Rosa JL, Vertommen D, Rider MH, Navarro-Sabate A, Bartrons R. Biochem J. 2013 Jun 15;452(3):531-43.

Functional metabolic screen identifies - Functional metabolic screen identifies

Ros S, Santos CR, Moco S, Baenke F, Kelly G, Howell M, Zamboni N, Schulze A. Cancer Discov. 2012 Apr;2(4):328-43.

Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation - Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation

Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J. J Biol Chem. 2009 Sep 4;284(36):24223-32.

Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and - Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and

Bobarykina AY, Minchenko DO, Opentanova IL, Moenner M, Caro J, Esumi H, Minchenko OH. Acta Biochim Pol. 2006;53(4):789-99. Epub 2006 Dec 4.

Hypoxia induces transcription of - Hypoxia induces transcription of

Minchenko O, Opentanova I, Minchenko D, Ogura T, Esumi H. FEBS Lett. 2004 Oct 8;576(1-2):14-20.

Hypoxic regulation of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase - Hypoxic regulation of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Minchenko O, Opentanova I, Caro J. FEBS Lett. 2003 Nov 20;554(3):264-70.