Anti-COG7 antibody produced in rabbit

Name	Anti-COG7 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200435
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human, Mouse, Rat
Antigen	peptide corresponding to an internal region of human COG7, conjugated to KLH
Description	Rabbit Polyclonal
Gene	COG7
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 1 μg/mL Rabbit Anti-COG7 (<B>Cat. No. SAB4200435</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (<B>Cat. No. 18772</B>). Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton^™ X-100. Fixed cells were stained with 1 μg/mL Rabbit Anti-COG7 (Cat. No. SAB4200435). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-COG7 (<B>Cat. No. SAB4200435</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/>Lanes<BR/>1. 2.5 μg/mL antibody<BR/>2. 5 μg/mL antibody<BR/>3. 5 μg/mL antibody + COG7 immunizing peptide

Immunoblotting
Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-COG7 (Cat. No. SAB4200435). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody
3. 5 μg/mL antibody + COG7 immunizing peptide

<B>Immunoprecipitation</B><BR/>Rabbit Anti-COG7 (<B>Cat. No. SAB4200435</B>) was used to immunoprecipitate COG7 from HeLa whole cell extract. Detection antibody: Rabbit Anti-COG7 (N-terminal) (<B>Cat. No. SAB4200436</B>).<BR/>Lanes<BR/>1. 5 μg antibody<BR/>2. 10 μg antibody<BR/>3. Negative control: 5 μg non-relevant antibody<BR/>4. Positive control: HeLa whole cell extract

Immunoprecipitation
Rabbit Anti-COG7 (Cat. No. SAB4200435) was used to immunoprecipitate COG7 from HeLa whole cell extract. Detection antibody: Rabbit Anti-COG7 (N-terminal) (Cat. No. SAB4200436).
Lanes
1. 5 μg antibody
2. 10 μg antibody
3. Negative control: 5 μg non-relevant antibody
4. Positive control: HeLa whole cell extract