ImmunofluorescenceHela cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 2 μg/mL Rabbit Anti-KTN1 (
Cat. No. K1769). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
ImmunoprecipitationRabbit Anti-KTN1 (
Cat. No. K1769) was used to immunoprecipitate KTN1 from Raji whole cell extract. Detection antibody: Rabbit Anti-KTN1 (C-terminal) antibody (
Cat. No. K1644).
Lanes1. 2 μg antibody
2. 5 μg antibody
3. Negative control: 5 μg non-relevant antibody
4. Negative control: without precipitating antibody
ImmunoblottingWhole extract of Hela cells was separated on SDS-PAGE and probed with Rabbit Anti-KTN1 (
Cat. No. K1769). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. 1 μg/mL antibody
2. 2 μg/mL antibody
3. 2 μg/mL + KTN1 immunizing peptide (human, 85-102)