Anti-CAP2 (116-130) antibody produced in rabbit

Name	Anti-CAP2 (116-130) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C7246
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 116-130 of human CAP2
Description	Rabbit Polyclonal
Gene	CAP2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-CAP2 (116-130): <B>Cat. No. C7246</B>: Immunofluorescence of HUVEC cells using CAP2 (116-130), Cat. No. C7246 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-CAP2 (116-130): Cat. No. C7246: Immunofluorescence of HUVEC cells using CAP2 (116-130), Cat. No. C7246 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CAP2 was separated on SDS-PAGE, blotted with Anti-CAP2 (116-130) (<B>Cat. No. C7246</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + CAP2 immunizing peptide (human, 116-130)

Immunoblotting
Lysate of HEK-293T cells overexpressing human CAP2 was separated on SDS-PAGE, blotted with Anti-CAP2 (116-130) (Cat. No. C7246) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + CAP2 immunizing peptide (human, 116-130)

<B>Chart</B><BR/>Anti-CAP2 (116-130): <B>Cat. No. C7246</B>: Intensity analysis of Anti-CAP2 (116-130) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-CAP2 (116-130): Cat. No. C7246: Intensity analysis of Anti-CAP2 (116-130) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Overexpression of cyclase-associated protein 2 in multistage - Overexpression of cyclase-associated protein 2 in multistage

Shibata R, Mori T, Du W, Chuma M, Gotoh M, Shimazu M, Ueda M, Hirohashi S, Sakamoto M. Clin Cancer Res. 2006 Sep 15;12(18):5363-8.

Involvement of hepatocellular carcinoma biomarker, cyclase-associated protein 2 - Involvement of hepatocellular carcinoma biomarker, cyclase-associated protein 2

Effendi K, Yamazaki K, Mori T, Masugi Y, Makino S, Sakamoto M. Exp Cell Res. 2013 Jan 1;319(1):35-44.

Mammalian CAP interacts with CAP, CAP2, and actin. - Mammalian CAP interacts with CAP, CAP2, and actin.

Hubberstey A, Yu G, Loewith R, Lakusta C, Young D. J Cell Biochem. 1996 Jun 1;61(3):459-66.

Comparison of human CAP and CAP2, homologs of the yeast adenylyl - Comparison of human CAP and CAP2, homologs of the yeast adenylyl

Yu G, Swiston J, Young D. J Cell Sci. 1994 Jun;107 ( Pt 6):1671-8.