Anti-CRAT (501-515) antibody produced in rabbit

Name	Anti-CRAT (501-515) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C7248
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 501-515 of human CRAT
Description	Rabbit Polyclonal
Gene	CRAT
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-CRAT (501-515): <B>Cat. No. C7248</B>: Immunofluorescence of HUVEC cells using CRAT (501-515), Cat. No. C7248 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-CRAT (501-515): Cat. No. C7248: Immunofluorescence of HUVEC cells using CRAT (501-515), Cat. No. C7248 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CRAT was separated on SDS-PAGE, blotted with Anti-CRAT (501-515) (<B>Cat. No. C7248</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + CRAT immunizing peptide (human, 501-515)

Immunoblotting
Lysate of HEK-293T cells overexpressing human CRAT was separated on SDS-PAGE, blotted with Anti-CRAT (501-515) (Cat. No. C7248) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + CRAT immunizing peptide (human, 501-515)

<B>Chart</B><BR/>Anti-CRAT (501-515): <B>Cat. No. C7248</B>: Intensity analysis of Anti-CRAT (501-515) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-CRAT (501-515): Cat. No. C7248: Intensity analysis of Anti-CRAT (501-515) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Structure of human carnitine acetyltransferase. Molecular basis for fatty acyl - Structure of human carnitine acetyltransferase. Molecular basis for fatty acyl

Wu D, Govindasamy L, Lian W, Gu Y, Kukar T, Agbandje-McKenna M, McKenna R. J Biol Chem. 2003 Apr 11;278(15):13159-65. Epub 2003 Jan 31.

Molecular cloning of cDNAs encoding human carnitine acetyltransferase and mapping - Molecular cloning of cDNAs encoding human carnitine acetyltransferase and mapping

Corti O, Finocchiaro G, Rossi E, Zuffardi O, DiDonato S. Genomics. 1994 Sep 1;23(1):94-9.

Substrate specificity of human carnitine acetyltransferase: Implications for - Substrate specificity of human carnitine acetyltransferase: Implications for

Violante S, Ijlst L, Ruiter J, Koster J, van Lenthe H, Duran M, de Almeida IT, Wanders RJ, Houten SM, Ventura FV. Biochim Biophys Acta. 2013 Jun;1832(6):773-9.

Carnitine insufficiency caused by aging and overnutrition compromises - Carnitine insufficiency caused by aging and overnutrition compromises

Noland RC, Koves TR, Seiler SE, Lum H, Lust RM, Ilkayeva O, Stevens RD, Hegardt FG, Muoio DM. J Biol Chem. 2009 Aug 21;284(34):22840-52.