ImmunoblottingCell lysates of HEK-293T overexpressing human Synip (Lanes 1,3) and of HEK-293T non-transfected (Lane 2), were separated on SDS-PAGE, blotted with Anti-Synip (N-terminal) (
Cat. No. S1700) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and chemiluminescent substrate.
Lanes1. Antibody: 0.15 μg/mL
2. Antibody: 0.15 μg/mL
3. Antibody: 0.15 μg/mL + Synip immunizing peptide (human, 58-76)
ImmunoblottingCell lysate of HepG2 (Lanes 1,2) was separated on SDS-PAGE, blotted with Anti-Synip (N-terminal) (
Cat. No. S1700) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and chemiluminescent substrate.
Lanes1. Antibody: 0.75 μg/mL
2. Antibody: 0.37 μg/mL