Anti-TIMM22 (96-110) antibody produced in rabbit

Name	Anti-TIMM22 (96-110) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	T8954
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 96-110 of human TIMM22
Description	Rabbit Polyclonal
Gene	TIMM22
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-TIMM22 (96-110): <B>Cat. No. T8954</B>: Immunofluorescence of HUVEC cells using TIMM22 (96-110), Cat. No. T8954 (red) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-TIMM22 (96-110): Cat. No. T8954: Immunofluorescence of HUVEC cells using TIMM22 (96-110), Cat. No. T8954 (red) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human TIMM22 was separated on SDS-PAGE, blotted with Anti-TIMM22 (96-110) (<B>Cat. No. T8954</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250<BR/>2. Antibody dilution 1:500 <BR/>3. Antibody dilution 1:500 + TIMM22 immunizing peptide (human, 96-110)

Immunoblotting
Lysate of HEK-293T cells overexpressing human TIMM22 was separated on SDS-PAGE, blotted with Anti-TIMM22 (96-110) (Cat. No. T8954) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Antibody dilution 1:500
3. Antibody dilution 1:500 + TIMM22 immunizing peptide (human, 96-110)

<B>Chart</B><BR/>Anti-TIMM22 (96-110): <B>Cat. No. T8954</B>: Intensity analysis of Anti-TIMM22 (96-110) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-TIMM22 (96-110): Cat. No. T8954: Intensity analysis of Anti-TIMM22 (96-110) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.