ImmunofluorescenceAnti-ACP2 (408-420) (RB):
Cat. No. A8232: Immunofluorescence of HUVEC cells using ACP2 (408-420) (RB),
Cat. No. A8232 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
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ImmunoblottingLysate of HEK-293T cells overexpressing human ACP2 was separated on SDS-PAGE, blotted with Anti-ACP2 (408-420) (
Cat. No. A8232) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes2. Antibody dilution 1:500
3. Antibody dilution 1:500 + ACP2 immunizing peptide (human, 408-420)
ChartAnti-ACP2 (408-420) (RB):
Cat. No. A8232: Intensity analysis of Anti-ACP2 (408-420) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.