Anti-MAGEA5 (76-87) antibody produced in rabbit

Name	Anti-MAGEA5 (76-87) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB1100976
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 76-87 of human MAGEA5
Description	Rabbit Polyclonal
Gene	MAGEA5
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>ANTI-MAGEA5 (76-87): <B>Cat. No. SAB1100976</B>: Immunofluorescence of HUVEC cells using MAGEA5 (76-87), Cat. No. SAB1100976 (red) at a 1:100 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
ANTI-MAGEA5 (76-87): Cat. No. SAB1100976: Immunofluorescence of HUVEC cells using MAGEA5 (76-87), Cat. No. SAB1100976 (red) at a 1:100 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human MAGEA5-FLAG Tag was separated on SDS-PAGE, blotted with Anti-MAGEA5 (76-87) (<B>Cat. No. SAB1100976</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250 <BR/>2. Negative control (only secondary antibody)<BR/>3. Anti-FLAG antibody (<B>Cat. No. F7425</B>), working concentration 2 μ/gL

Immunoblotting
Lysate of HEK-293T cells overexpressing human MAGEA5-FLAG Tag was separated on SDS-PAGE, blotted with Anti-MAGEA5 (76-87) (Cat. No. SAB1100976) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Negative control (only secondary antibody)
3. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μ/gL

<B>Chart</B><BR/>ANTI-MAGEA5 (76-87): <B>Cat. No. SAB1100976</B>: Intensity analysis of ANTI-MAGEA5 (76-87)staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
ANTI-MAGEA5 (76-87): Cat. No. SAB1100976: Intensity analysis of ANTI-MAGEA5 (76-87)staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6400) as compared with the negative control. Yale HTCB IF procedure used.