Anti-ATP6V1F (46-60) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: SAB1100954
• Prices: $328.50
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 46-60 of human ATP6V1F
• Description: Rabbit Polyclonal
• Gene: ATP6V1F
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-ATP6V1F (46-60) (RB): Cat. No. SAB1100954: Immunofluorescence of HUVEC cells using Anti-ATP6V1F (46-60) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence Graph
Anti-ATP6V1F (46-60) (RB): Cat. No. SAB1100954: Intensity analysis of Anti-ATP6V1F (46-60) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6400) as compared with the negative control.

Immunoblotting
Lysate of HEK-293T cells overexpressing human ATP6V1F was separated on SDS-PAGE, blotted with Anti-ATP6V1F (46-60) (Cat. No. SAB1100954) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Antibody dilution 1:250 + ATP6V1F immunizing peptide (human, 46-60)