Monoclonal Anti-Grp78 - Atto 655 antibody produced in mouse

Name	Monoclonal Anti-Grp78 - Atto 655 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB5200174
Prices	$395.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1κ
Clone	3C5-1A4
Applications	ELISA WB
Species Reactivities	Mouse, Rat, Human
Antigen	Full-length recombinant rat GRP78
Description	Mouse Monoclonal
Gene	Hspa5
Conjugate	Atto 655 conjugate
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Monoclonal Anti-GRP78: Western blot detection of ~78 kDa GRP78 protein using GRP78 antibody, clone 3C5-1A4. Load: 20 μg lysate per lane. Block: 5% milk + TBST 60 min at RT. Primary antibody: Mouse Anti-GRP78, clone 3C5-1A4, incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody for 60 min at RT (1:50/blocking buffer). Color Development: TMB solution for 5 min at RT. Predicted/Observed size: ~78 kDa. <BR/><B>Lanes</B><BR/>1. MW ladder<BR/>2. Human HEK-293<BR/>3. Mouse NIH3T3<BR/>4. Rat Brain

Immunoblotting
Monoclonal Anti-GRP78: Western blot detection of ~78 kDa GRP78 protein using GRP78 antibody, clone 3C5-1A4. Load: 20 μg lysate per lane. Block: 5% milk + TBST 60 min at RT. Primary antibody: Mouse Anti-GRP78, clone 3C5-1A4, incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody for 60 min at RT (1:50/blocking buffer). Color Development: TMB solution for 5 min at RT. Predicted/Observed size: ~78 kDa.
Lanes
1. MW ladder
2. Human HEK-293
3. Mouse NIH3T3
4. Rat Brain

Representative absorption and emission spectra chart of ATTO 655 dye