Anti-DLX1 (96-109) antibody produced in rabbit

Name	Anti-DLX1 (96-109) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	D8070
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 96-109 of human DLX1
Description	Rabbit Polyclonal
Gene	DLX1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-DLX1 (96-109): <B>Cat. No. D8070</B>: Immunofluorescence of HUVEC cells using DLX1 (96-109), Cat. No. D8070 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-DLX1 (96-109): Cat. No. D8070: Immunofluorescence of HUVEC cells using DLX1 (96-109), Cat. No. D8070 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DLX1 was separated on SDS-PAGE, blotted with Anti-DLX1 (96-109) (<B>Cat. No. D8070</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + DLX1 immunizing peptide (human, 96-109)

Immunoblotting
Lysate of HEK-293T cells overexpressing human DLX1 was separated on SDS-PAGE, blotted with Anti-DLX1 (96-109) (Cat. No. D8070) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + DLX1 immunizing peptide (human, 96-109)

<B>Chart</B><BR/>Anti-DLX1 (96-109): <B>Cat. No. D8070</B>: Intensity analysis of Anti-DLX1 (96-109) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-DLX1 (96-109): Cat. No. D8070: Intensity analysis of Anti-DLX1 (96-109) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

The DLX1and DLX2 genes and susceptibility to autism spectrum disorders. - The DLX1and DLX2 genes and susceptibility to autism spectrum disorders.

Liu X, Novosedlik N, Wang A, Hudson ML, Cohen IL, Chudley AE, Forster-Gibson CJ, Lewis SM, Holden JJ. Eur J Hum Genet. 2009 Feb;17(2):228-35.

Homeoprotein DLX-1 interacts with Smad4 and blocks a signaling pathway from - Homeoprotein DLX-1 interacts with Smad4 and blocks a signaling pathway from

Chiba S, Takeshita K, Imai Y, Kumano K, Kurokawa M, Masuda S, Shimizu K, Nakamura S, Ruddle FH, Hirai H. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15577-82. Epub 2003 Dec 11.

Genomic structure and functional control of the Dlx3-7 bigene cluster. - Genomic structure and functional control of the Dlx3-7 bigene cluster.

Sumiyama K, Irvine SQ, Stock DW, Weiss KM, Kawasaki K, Shimizu N, Shashikant CS, Miller W, Ruddle FH. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):780-5. Epub 2002 Jan 15.