Anti-DBP (216-228) antibody produced in rabbit

Name	Anti-DBP (216-228) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	D6320
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 216-228 of human DBP
Description	Rabbit Polyclonal
Gene	DBP
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-DBP (216-228): <B>Cat. No. D6320</B>: Immunofluorescence of HUVEC cells using DBP (216-228), Cat. No. D6320 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-DBP (216-228): Cat. No. D6320: Immunofluorescence of HUVEC cells using DBP (216-228), Cat. No. D6320 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DBP was separated on SDS-PAGE, blotted with Anti-DBP (216-228) (<B>Cat. No. D6320</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + DBP immunizing peptide (human, 216-228)<BR/>3. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human DBP was separated on SDS-PAGE, blotted with Anti-DBP (216-228) (Cat. No. D6320) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + DBP immunizing peptide (human, 216-228)
3. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-DBP (216-228): <B>Cat. No. D6320</B>: Intensity analysis of Anti-DBP (216-228) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-DBP (216-228): Cat. No. D6320: Intensity analysis of Anti-DBP (216-228) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

The ins and outs of circadian timekeeping. - The ins and outs of circadian timekeeping.

Brown SA, Schibler U. Curr Opin Genet Dev. 1999 Oct;9(5):588-94.

The circadian PAR-domain basic leucine zipper transcription factors DBP, TEF, and - The circadian PAR-domain basic leucine zipper transcription factors DBP, TEF, and

Gachon F, Olela FF, Schaad O, Descombes P, Schibler U. Cell Metab. 2006 Jul;4(1):25-36.

CLOCK, an essential pacemaker component, controls expression of the circadian - CLOCK, an essential pacemaker component, controls expression of the circadian

Ripperger JA, Shearman LP, Reppert SM, Schibler U. Genes Dev. 2000 Mar 15;14(6):679-89.

Genomic structure of the human D-site binding protein (DBP) gene. - Genomic structure of the human D-site binding protein (DBP) gene.

Shutler G, Glassco T, Kang X, Korneluk R, Mueller CR. Genomics. 1996 Jun 15;34(3):334-9.