Monoclonal Anti-RABL2A antibody produced in mouse

Name	Monoclonal Anti-RABL2A antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4100159
Prices	$358.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	RABL2A-A127
Applications	WB
Species Reactivities	Human
Antigen	RABL2A purified recombinant protein
Description	Mouse Monoclonal
Gene	RABL2A
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HT29 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-RABL2A Clone: RABL2A-A127 (<B>Cat. No. SAB4100159</B>) diluted to 1:25. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HT29 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-RABL2A Clone: RABL2A-A127 (Cat. No. SAB4100159) diluted to 1:25. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells expressing FLAG<SUP>®</SUP>-tagged human RABL2A was separated on SDS-PAGE, probed with Mouse Anti-RABL2A Clone: RABL2A-A127 (<B>Cat. No. SAB4100159</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:1,000<BR/>3. Negative control<BR/>4. Positive control Anti-FLAG<SUP>®</SUP>

Immunoblotting
Lysate of HEK-293T cells expressing FLAG^®-tagged human RABL2A was separated on SDS-PAGE, probed with Mouse Anti-RABL2A Clone: RABL2A-A127 (Cat. No. SAB4100159). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:1,000
3. Negative control
4. Positive control Anti-FLAG^®

<B>Immunoblotting</B><BR/>HT29 cell extract was separated on SDS-PAGE and probed with Mouse Anti-RABL2A Clone: RABL2A-A127 (<B>Cat. No. SAB4100159</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Negative control

Immunoblotting
HT29 cell extract was separated on SDS-PAGE and probed with Mouse Anti-RABL2A Clone: RABL2A-A127 (Cat. No. SAB4100159). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Negative control

<B>Flow Cytometry</B><BR/>1×106 HT29 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with Mouse Anti-RABL2A Clone: RABL2A-A127 (<B>Cat. No. SAB4100159</B>) diluted to 1:10 followed by Rabbit Anti-Mouse IgG, FITC conjugate. Shaded histogram: negative control without first antibody, Green histogram: antibody specific staining.

Flow Cytometry
1×106 HT29 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with Mouse Anti-RABL2A Clone: RABL2A-A127 (Cat. No. SAB4100159) diluted to 1:10 followed by Rabbit Anti-Mouse IgG, FITC conjugate. Shaded histogram: negative control without first antibody, Green histogram: antibody specific staining.