ImmunofluorescenceHela cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 2 μg/mL Rabbit Anti-KTN1 (C-terminal) (
Cat. No. K1644). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
ImmunoprecipitationRabbit Anti-KTN1 (C-terminal) (
Cat. No. K1644) was used to immunoprecipitate KTN1 from Jurkat whole cell extract. Detection antibody: Rabbit Anti-KTN1 antibody (
Cat. No. K1769).
Lanes1. 5 μg antibody
2. Negative control: 5 μg non-relevant antibody
ImmunoblottingWhole extract of Jurkat cells was separated on SDS-PAGE and probed with Rabbit Anti-KTN1 (C-terminal) (
Cat. No. K1644). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. 2 μg/mL antibody
2. 4 μg/mL antibody
3. 4 μg/mL + KTN1 immunizing peptide (human, 1278-1295)