Monoclonal Anti-PYCRL antibody produced in mouse

Name	Monoclonal Anti-PYCRL antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4100231
Prices	$339.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	PYCRL-A189
Applications	WB
Species Reactivities	Human
Antigen	PYCRL purified recombinant protein
Description	Mouse Monoclonal
Gene	PYCRL
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>MCF7 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-PYCRL Clone: PYCRL-A189 (<B>Cat. No. SAB4100231</B>) diluted to 1:20. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
MCF7 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-PYCRL Clone: PYCRL-A189 (Cat. No. SAB4100231) diluted to 1:20. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells expressing FLAG<SUP>®</SUP>-tagged human PYCRL was separated on SDS-PAGE, probed with Mouse Anti-PYCRL Clone: PYCRL-A189 (<B>Cat. No. SAB4100231</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:2,000<BR/>2. Negative control<BR/>3. Positive control Anti-FLAG<SUP>®</SUP>

Immunoblotting
Lysate of HEK-293T cells expressing FLAG^®-tagged human PYCRL was separated on SDS-PAGE, probed with Mouse Anti-PYCRL Clone: PYCRL-A189 (Cat. No. SAB4100231). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:2,000
2. Negative control
3. Positive control Anti-FLAG^®

<B>Immunoblotting</B><BR/>MCF7 cell extract was separated on SDS-PAGE and probed with Mouse Anti-PYCRL Clone: PYCRL-A189 (<B>Cat. No. SAB4100231</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:50<BR/>2. Antibody dilution 1:100<BR/>3. Negative control

Immunoblotting
MCF7 cell extract was separated on SDS-PAGE and probed with Mouse Anti-PYCRL Clone: PYCRL-A189 (Cat. No. SAB4100231). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:50
2. Antibody dilution 1:100
3. Negative control