ImmunoblottingLysate of HEK-293T cells overexpressing human RCBTB2 was separated on SDS-PAGE, blotted with Anti-RCBTB2 (186-200) (
Cat. No. R6657) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:250
ImmunofluorescenceAnti-RCBTB2 (186-200):
Cat. No. R6657: Immunofluorescence of HUVEC cells using RCBTB2 (186-200), Cat. No. R6657 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ChartAnti-RCBTB2 (186-200):
Cat. No. R6657: Intensity analysis of Anti-RCBTB2 (186-200) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.