Anti-Raver1 (N-terminal) antibody produced in rabbit

Name	Anti-Raver1 (N-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200091
Prices	$61.10, $360.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Antigen	synthetic peptide corresponding to amino acids 29-45 of human Raver1 conjugated to KLH
Description	Rabbit Polyclonal
Gene	RAVER1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Nuclear localization of Raver1 in HEK-293T cells. Cells were fixed and stained with 5 μg/mL Rabbit Anti-Raver1 (N-Terminal) (<B>Cat. No. SAB4200091</B>) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (×40 magnification).

Immunofluorescence
Nuclear localization of Raver1 in HEK-293T cells. Cells were fixed and stained with 5 μg/mL Rabbit Anti-Raver1 (N-Terminal) (Cat. No. SAB4200091) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (×40 magnification).

<B>Immunoblotting</B><BR/>Lysates of the indicated cells were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-Raver1 (N-Terminal) (<B>Cat. No. SAB4200091</B>). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (<B>Cat. No. A0545</B>) and a chemiluminescence substrate. <BR/><B>Lanes</B><BR/>1. 293T; 2. HepG2; and 3. K-562

Immunoblotting
Lysates of the indicated cells were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-Raver1 (N-Terminal) (Cat. No. SAB4200091). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate.
Lanes
1. 293T; 2. HepG2; and 3. K-562

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Raver1 (N-Terminal) (<B>Cat. No. SAB4200091</B>) was used to immunoprecipitate human Raver1 from HEK-293T cells. Detection antibody: Anti-Raver1 (N-Terminal) (<B>Cat. No. SAB4200091</B>). <BR/><B>Lanes</B><BR/>1. IP antibody: 5 μg<BR/>2. IP antibody:10 μg<BR/>3. Negative control: IP antibody without cell lysate<BR/>4. Negative control: without IP antibody<BR/>5. Immunoblotting positive control: total HEK-293T cell lysate

Immunoprecipitation
Rabbit Anti-Raver1 (N-Terminal) (Cat. No. SAB4200091) was used to immunoprecipitate human Raver1 from HEK-293T cells. Detection antibody: Anti-Raver1 (N-Terminal) (Cat. No. SAB4200091).
Lanes
1. IP antibody: 5 μg
2. IP antibody:10 μg
3. Negative control: IP antibody without cell lysate
4. Negative control: without IP antibody
5. Immunoblotting positive control: total HEK-293T cell lysate