ImmunoblottingLysate of HEK-293T cells overexpressing human FMO4 was separated on SDS-PAGE, blotted with Anti-FMO4 (71-85) (
Cat. No. F2182) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:500 + FMO4 immunizing peptide (human, 71-85)
ImmunofluorescenceAnti-FMO4 (71-85):
Cat. No. F2182: Immunofluorescence of HUVEC cells using FMO4 (71-85), Cat. No. F2182 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ChartAnti-FMO4 (71-85):
Cat. No. F2182: Intensity analysis of Anti-FMO4 (71-85) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.