Immunoblotting Lysate of HEK-293T cells over-expressing Nezha was separated on SDS-PAGE and probed with Rabbit Anti-Nezha (N-Terminal) (
Cat. No. SAB4200172). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (
Cat. No. A0545) and a chemiluminescence substrate.
Lanes1. Antibody: 4 μg/mL
2. Antibody: 2 μg/mL
3. Antibody: 1 μg/mL
4. Antibody: 0.5 μg/mL
5. Antibody: 2 μg/mL + 10 μg/mL Nezha immunizing peptide
ImmunoprecipitationRabbit Anti-Nezha (N-Terminal) (
Cat. No. SAB4200172) was used to immunoprecipitate Nezha from Lysate of HEK-293Tcells over-expressing human Nezha. Detection Antibody Rabbit Anti-Nezha (C-Terminal) (
Cat. No. SAB4200171).
Lanes1. IP antibody: 10 μg
2. IP antibody: 5 μg
3. IP antibody: 2.5 μg
4. Negative control: without IP antibody
5. Negative control: IP antibody without cell lysate