ImmunohistochemistryLocalization of dystrobrevin-β at renal tubules in rat kidney. Formalin-fixed paraffin-embedded tissue was stained with 20 μg/mL Anti-dystrobrevin-β (
Cat. No. SAB4200288) followed by Goat Anti-Rabbit IgG, HRP conjugate (×20 magnification).
ImmunofluorescenceCytoplasmic localization of dystrobrevin-β in HEK-293T cells overexpressing human dystrobrevin-β. Fixed cells were stained with 3 μg/mL Anti-Dystrobrevin-β (
Cat. No. SAB4200288) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunoblottingCell lysates of HEK293-T overexpressing human dystrobrevin-β (lanes 1,3), HEK293-T non-transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-Dystrobrevin-β (
Cat. No. SAB4200288), and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody: 1.5 μg/mL
2. Antibody: 1.5 μg/mL
3. Antibody: 1.5 μg/mL + dystrobrevin-β immunizing peptide
ImmunoprecipitationAnti-Dystrobrevin-β (
Cat. No. SAB4200288), was used to immunoprecipitate dystrobrevin-β from HEK293-T overexpressing human dystrobrevin-β. Detection antibody: Anti-Dystrobrevin-β (N-terminal) (
Cat. No. SAB4200289),
Lanes1. IP antibody: 15 μg
2. Negative control: without cell lysate
3. Negative control: without IP antibody