ImmunohistochemistryLocalization of tensin 1 at sarcomeric fibers in rat heart. Formalin-fixed paraffin-embedded tissue was stained with 10 μg/mL Anti-Tensin 1 (
Cat. No. SAB4200283) followed by Goat Anti-Rabbit IgG, HRP conjugate (×20 magnification).
ImmunofluorescenceCytoplasmic and focal adhesion localization of tensin 1 in chicken embryo fibroblast cells. Fixed cells were stained with 3 μg/mL Anti-Tensin 1 (
Cat. No. SAB4200283) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunoblottingCell and tissue extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-Tensin 1 (
Cat. No. SAB4200283), and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. A10
2. Rat heart
3. HEK293-T overexpressing human tensin 1
4. HEK293-T non-transfected
5. A10: Antibody + immunizing tensin 1 peptide
6. HEK293-T overexpressing human tensin 1. Antibody + immunizing tensin 1 peptide