Anti-COG1 (C-TERMINAL) antibody produced in rabbit
| Name | Anti-COG1 (C-TERMINAL) antibody produced in rabbit |
|---|---|
| Supplier | Sigma-Aldrich |
| Catalog | SAB4200424 |
| Prices | $337.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | peptide corresponding to the C-terminal region of human COG1, conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | COG1 |
| Conjugate | unconjugated |
| Supplier Page | Shop |
Product images
ImmunofluorescenceHeLa cells were fixed and permeabilized with 1% paraformaldehyde in a buffer containing 10 mM MES pH 6.2, 10 mM NaCl, 1.5 mM MgCl2 and 2.5% glycerol and subsequently permeabilized with 0.1% Triton™ X-100. Fixed cells were co-immunostained with 2 μg Rabbit Anti-COG1 (C-terminal) (Cat. No. SAB4200424) followed by Goat Anti-Rabbit IgG, Cy3™ conjugate (red, panel A and B) and with a Golgi marker (green, panel B). Cells were counterstained with DAPI to stain nuclei (blue, panel B). The pictures are courtesy of Dr. Orly Laufman and Prof. Sima Lev, Dept. of Molecular Cell Biology, Weizmann institute of Science, Rehovot, Israel.
ImmunofluorescenceHeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton™ X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-COG1 (C-terminal) (Cat. No. SAB4200424). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei. Magnification X100.
ImmunofluorescenceHeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton™ X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-COG1 (C-terminal) (Cat. No. SAB4200424). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei. Magnification X40.
ImmunoblottingWhole extract of HepG2 cells was separated on SDS-PAGE and probed with Rabbit Anti-COG1 (C-terminal) (Cat. No. SAB4200424). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody
3. 5 μg/mL antibody + COG1 (C-terminal) immunizing peptide
ImmunoblottingWhole extract of LA4 cells was separated on SDS-PAGE and probed with Rabbit Anti-COG1 (C-terminal) (Cat. No. SAB4200424). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2.5 μg/mL antibody
2. 5 μg/mL antibody
3. 5 μg/mL antibody + COG1 (C-terminal) immunizing peptide