ImmunofluorescenceHepG2 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-NT5DC1 Clone: NT5DC1-B165 (
Cat. No. SAB4100268) diluted to 1:50. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingLysate of HEK-293T cells expressing FLAG
®-tagged human NT5DC1 was separated on SDS-PAGE, probed with Mouse Anti-NT5DC1 Clone: NT5DC1-B165 (
Cat. No. SAB4100268). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:2,000
2. Negative control
3. Positive control Anti-FLAG
®
ImmunoblottingHepG2 cell extract was separated on SDS-PAGE and probed with Mouse Anti-NT5DC1 Clone: NT5DC1-B165 (
Cat. No. SAB4100268). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Negative control